Dual RMCE for efficient re-engineering of mouse mutant alleles.
Nat Methods
; 7(11): 893-5, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-20953177
ABSTRACT
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ingeniería Genética
/
Recombinasas
/
Células Madre Embrionarias
Límite:
Animals
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2010
Tipo del documento:
Article
País de afiliación:
Suiza