Intrinsic phospholipase A2 activity of adeno-associated virus is involved in endosomal escape of incoming particles.
Virology
; 409(1): 77-83, 2011 Jan 05.
Article
en En
| MEDLINE
| ID: mdl-20974479
The unique region of the VP1 capsid protein of adeno-associated viruses (AAV) in common with autonomously replicating parvoviruses comprises a secreted phospholipase A2 (sPLA2) homology domain. While the sPLA2 domain of Minute Virus of Mice has recently been shown to mediate endosomal escape by lipolytic pore formation, experimental evidence for a similar function in AAV infection is still lacking. Here, we explored the function of the sPLA2 domain of AAV by making use of the serotype 2 mutant (76)HD/AN. The sPLA2 defect in (76)HD/AN, which severely impairs AAV's infectivity, could be complemented in trans by co-infection with wild-type AAV2. Furthermore, co-infection with endosomolytically active, but not with inactive adenoviral variants partially rescued (76)HD/AN, providing the first evidence for a function of this domain in endosomal escape of incoming AAV particles.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Endosomas
/
Virión
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Dependovirus
/
Proteínas de la Cápside
/
Fosfolipasas A2
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania