Construction of a genetic AND gate under a new standard for assembly of genetic parts.
BMC Genomics
; 11 Suppl 4: S16, 2010 Dec 02.
Article
en En
| MEDLINE
| ID: mdl-21143799
ABSTRACT
BACKGROUND:
Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.RESULTS:
We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.CONCLUSIONS:
We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Secuencias Reguladoras de Ácidos Nucleicos
/
Biología Computacional
Idioma:
En
Revista:
BMC Genomics
Asunto de la revista:
GENETICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón