Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145.
Appl Microbiol Biotechnol
; 89(4): 1149-59, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21229241
ABSTRACT
GlnR is the global regulator of nitrogen assimilation in Streptomyces coelicolor M145 and other actinobacteria. Two-dimensional polyacrylamide gel electrophoresis analyses were performed to identify new GlnR target genes by proteomic comparison of wild-type S. coelicolor M145 and a ΔglnR mutant. Fifty proteins were found to be differentially regulated between S. coelicolor M145 and the ΔglnR mutant. These spots were identified by nanoHPLC-ESI-MS/MS and classified according to their cellular role. Most of the identified proteins are involved in amino acid biosynthesis and in carbon metabolism, demonstrating that the role of GlnR is not restricted to nitrogen metabolism. Thus, GlnR is supposed to play an important role in the global metabolic control of S. coelicolor M145.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Regulación Bacteriana de la Expresión Génica
/
Transactivadores
/
Proteoma
/
Streptomyces coelicolor
/
Nitrógeno
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania