Investigation of the effects of the chronic administration of some antihypertensive drugs on enzymatic and non-enzymatic oxidant/antioxidant parameters in rat ovarian tissue.

ABSTRACT

OBJECTIVE: In this study, effects of chronic antihypertensive drug (clonidine, methyldopa, amlodipine, ramipril, and rilmenidine) treatment on antioxidant-oxidant parameters were investigated in rat ovarian tissue. STUDY DESIGN: Chronic drug administration for 30 days and at the end, biochemical examinations (total glutathione (tGSH), glutathione peroxidase (GPO), glutathione reductase (GR), glutathione s-transferase (GST), superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO) analyses) were performed. RESULTS: The levels of glutathione (GSH) and NO, and the activities of GPO, GR, GST, SOD, and CAT were measured the lowest in ramiprile group. Also in ramiprile group, the level of MDA and the activity of MPO was the highest. CONCLUSION: We divided the drugs into four groups according to their biochemical side effect potentials in ovarian tissue (I) Drugs which have no clear negative effect on ovarian tissue clonidine, rilmenidine; (II) Drugs which have mild negative effect on ovarian tissue methyldopa; (III) Drugs which have moderate negative effect on ovarian tissue amlodipine; (IV) Drugs which have severe negative effect on ovarian tissue ramipril. These data might be useful in the selection of the least toxic antihypertensive drug in pregnant and/or normal females.