[Detection of multiple Ophiocordyceps sinensis mutants in premature stroma of Cordyceps sinensis by MassARRAY SNP MALDI-TOF mass spectrum genotyping].
Beijing Da Xue Xue Bao Yi Xue Ban
; 43(2): 259-66, 2011 Apr 18.
Article
en Zh
| MEDLINE
| ID: mdl-21503123
ABSTRACT
OBJECTIVE:
To examine the mutants of Ophiocordyceps sinensis (Os) in the stroma of premature Cordyceps sinensis (Cs).METHODS:
Used MassARRAY single nucleotide polymorphism (SNP) MALDI-TOF mass spectrum genotyping, designed eight SNP extension primers on the basis of the scattered, multiple point mutations of known Os mutants within their internal transcribed spacer (ITS) segments, and examined the Os mutant genotypes relating to the GC-biased Os genotype (gb #AB067721) in premature Cs stroma.RESULTS:
The two AT-biased genotypes and the GC-biased Os were simultaneously detected in premature Cs stroma. SNP genotyping also detected at least two other Os genotypes of unknown sequences.CONCLUSION:
Coexistence of the three known Os genotypes indicates the existence of possible transition point mutations within Os genes during germination and early maturation of Cs. Simultaneous detection of at least two unknown genotypes coexisting with those known mutants possibly evidences the transversion mutations within Os genes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Mutación Puntual
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Polimorfismo de Nucleótido Simple
/
Cordyceps
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Beijing Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2011
Tipo del documento:
Article
País de afiliación:
China