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Immortalized mouse embryo fibroblasts are resistant to miR-290-induced senescence regardless of p53 status.
Rizzo, Milena; Evangelista, Monica; Mariani, Laura; Simili, Marcella; Rainaldi, Giuseppe; Pitto, Letizia.
Afiliación
  • Rizzo M; Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, Pisa, Italy.
Physiol Genomics ; 43(20): 1153-9, 2011 Oct 20.
Article en En | MEDLINE | ID: mdl-21846807
ABSTRACT
The prosenescence role of miR-290 and nocodazole has been documented in primary mouse embryo fibroblasts (MEF), while it is not clear whether immortal murine fibroblasts are still responsive to these senescence inducing stimuli. To establish this point, immortal murine fibroblasts with functional (NIH3T3) or nonfunctional p53 (I-MEF) and low levels of miR-290 were tested for their capability to undergo senescence after exposure to either nocodazole or miR-290. Our results clearly indicate that nocodazole induces senescence only in NIH3T3 cells with a functional p53 but not in I-MEF lacking a functional p53. miR-290 overexpression is unable to address any of the tested immortalized clones toward senescence, regardless of the p53 status, suggesting that the prosenescence role of miR-290 is specific for primary but not for immortal murine fibroblasts. Moreover our findings suggest that the mere downregulation of a potential tumor suppressor miRNA in a given cell type does not necessarily imply that it behaves as a tumor suppressor.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteína p53 Supresora de Tumor / Senescencia Celular / MicroARNs / Embrión de Mamíferos / Fibroblastos Límite: Animals Idioma: En Revista: Physiol Genomics Asunto de la revista: BIOLOGIA MOLECULAR Año: 2011 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteína p53 Supresora de Tumor / Senescencia Celular / MicroARNs / Embrión de Mamíferos / Fibroblastos Límite: Animals Idioma: En Revista: Physiol Genomics Asunto de la revista: BIOLOGIA MOLECULAR Año: 2011 Tipo del documento: Article País de afiliación: Italia