Identification of clinical isolates of α-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison.
J Clin Microbiol
; 50(12): 4087-90, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22993176
ABSTRACT
Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones Estreptocócicas
/
Streptococcus
/
Técnicas de Tipificación Bacteriana
/
Análisis de Secuencia de ADN
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Reino Unido