Millimeter scale separation of DNA with a replaceable polymer matrix.
Electrophoresis
; 33(21): 3213-21, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-23027089
ABSTRACT
Electrophoresis is a powerful method that has seen a wide range of applications, often in automated genetic diagnostic instruments that require the use of a replaceable sieving matrix. The power and simplicity of electrophoresis as an analysis technique would be ideal for highly integrated and low-cost analysis systems if the method could be implemented in microfluidics on the scale of several mm. We demonstrate the electrophoretic analysis of DNA with separation lengths as small as 2 mm and with a resolution adequate for the analysis of PCR products, i.e. resolutions of 10-20 base pairs. Such small-scale separations enable analysis systems consisting of microfluidics and microelectronics integrated into a single inexpensive package, thereby overcoming a key challenge facing the development of the lab on chip technologies.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Resinas Acrílicas
/
ADN
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Electroforesis por Microchip
Idioma:
En
Revista:
Electrophoresis
Año:
2012
Tipo del documento:
Article
País de afiliación:
Canadá