Selective RNase H cleavage of target RNAs from a tRNA scaffold.
Methods Mol Biol
; 941: 9-18, 2012.
Article
en En
| MEDLINE
| ID: mdl-23065550
ABSTRACT
In vivo overproduction of tRNA chimeras yields an RNA insert within a tRNA scaffold. For some applications, it may be necessary to discard the scaffold. Here we present a protocol for selective cleavage of the RNA of interest from the tRNA scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN de Transferencia
/
ARN Ribosómico 16S
/
Ribonucleasa H
/
División del ARN
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2012
Tipo del documento:
Article
País de afiliación:
Francia