Molecular cloning and characterization of an endogenous digestive ß-glucosidase from the midgut of the fungus-growing termite Macrotermes barneyi.
Insect Mol Biol
; 21(6): 604-14, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-23126269
ABSTRACT
ß-glucosidase from the midgut of the fungus-growing termite Macrotermes barneyi was first cloned and characterized to gain a better understanding of cellulolytic systems in fungus-growing termites. ß-glucosidase activity was proven to present primarily in the midgut of M. barneyi and two ß-glucosidases were partially purified from the midgut. Based on the N-terminus sequence of one of the ß-glucosidases, a full-length cDNA fragment of 1708 bp was obtained. This sequence encodes a 493 amino acid protein belonging to glycoside hydrolase family 1. Quantitative real-time PCR analysis proved that the ß-glucosidase gene was primarily expressed in the midgut. ß-glucosidase was expressed heterologously and biochemically characterized. Results indicate that ß-glucosidase is an endogenous, midgut-origin termite digestive enzyme. It may have applications in understanding the mechanism of lignocellulose degradation in fungus-growing termites.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Beta-Glucosidasa
/
Proteínas de Insectos
/
Isópteros
Límite:
Animals
Idioma:
En
Revista:
Insect Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2012
Tipo del documento:
Article
País de afiliación:
China