R164H and V240H replacements by site-directed mutagenesis of TEM-149 extended-spectrum ß-lactamase: kinetic analysis of TEM-149H240 and TEM-149H164-H240 laboratory mutants.
Antimicrob Agents Chemother
; 57(2): 1047-9, 2013 Feb.
Article
en En
| MEDLINE
| ID: mdl-23183431
ABSTRACT
Two laboratory mutant forms, TEM-149(H240) and TEM-149(H164-H240), of the TEM-149 extended-spectrum ß-lactamase enzyme were constructed by site-directed mutagenesis. TEM-149(H240) and TEM-149(H164-H240) were similar in kinetic behavior, except with respect to benzylpenicillin and ceftazidime. Molecular modeling of the two mutant enzymes demonstrated the role of histidine at position 240 in the reduction of the affinity of the enzyme for ceftazidime.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Penicilina G
/
Beta-Lactamasas
/
Ceftazidima
/
Antibacterianos
Idioma:
En
Revista:
Antimicrob Agents Chemother
Año:
2013
Tipo del documento:
Article
País de afiliación:
Italia