Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging.

Tanaka, Torahiko; Kuroda, Kazumichi; Ikeda, Masanori; Kato, Nobuyuki; Shimizu, Kazufumi; Makishima, Makoto

Tanaka, Torahiko; Kuroda, Kazumichi; Ikeda, Masanori; Kato, Nobuyuki; Shimizu, Kazufumi; Makishima, Makoto.

Afiliación

Tanaka T; Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address: tanaka.torahiko@nihon-u.ac.jp.

J Biosci Bioeng ; 116(5): 620-3, 2013 Nov.

Article en En | MEDLINE | ID: mdl-23742748

ABSTRACT

ABSTRACT

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

Asunto(s)

Hepacivirus/química; Lípidos; Proteínas/química; Proteínas/metabolismo; Línea Celular; Supervivencia Celular; Humanos; Proteínas de la Membrana/metabolismo; Microscopía Confocal; Perilipina-2; Estructura Terciaria de Proteína; Proteínas/genética; Especificidad por Sustrato; Factores de Tiempo; Proteínas no Estructurales Virales/química

Palabras clave

Adipose differentiation-related protein; Hepatitis C virus; Lipid droplets; Nonstructural protein 4B; Time-lapse imaging

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas / Hepacivirus / Lípidos Límite: Humans Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2013 Tipo del documento: Article

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