Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model.
J Infect Dis
; 209(9): 1354-61, 2014 May 01.
Article
en En
| MEDLINE
| ID: mdl-24415790
ABSTRACT
BACKGROUND:
Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear.METHODS:
We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus.RESULTS:
Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells.CONCLUSIONS:
Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Gripe Humana
/
Subtipo H1N1 del Virus de la Influenza A
/
Anticuerpos Antivirales
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
J Infect Dis
Año:
2014
Tipo del documento:
Article
País de afiliación:
Reino Unido