Preparation of cell blocks for lung cancer diagnosis and prediction: protocol and experience of a high-volume center.
Respiration
; 87(5): 432-8, 2014.
Article
en En
| MEDLINE
| ID: mdl-24457174
ABSTRACT
Minimally invasive diagnostic techniques are increasingly being used to obtain specimens for pathological diagnosis and prediction. Referring to lung cancer, both endobronchial and endoesophageal ultrasound are used worldwide as diagnostic routine methods. Consequently, an increasing number of pathological samples are cytological and fewer are histological. On the other hand, the requirements for specific and sensitive tumor subtyping complemented by predictive analyses are steadily increasing and are an essential basis for evidence-based treatment decisions. In this article we focus on the cell block method as a helpful tool for diagnostic and predictive analyses in lung cancer and point out its advantages and disadvantages in comparison to conventional cytological and biopsy specimens. Furthermore, we retrospectively analyze the diagnostic results of the cell block method in a high-volume center over 5 years. The main advantages of cell blocks are the availability of established and validated protocols, archiving and the opportunity to have serial sections from the same specimens to provide or repeat molecular analyses. Actually, in case of tumor progression, even additional biomarkers can be tested using the original cell block when re-biopsies are not feasible. The cell block method should be considered as a reliable, complimentary approach to conventional cytological or biopsy procedures, which is helpful to fulfill the increasing requirements of high-quality diagnostics and prediction.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
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Biopsia
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Biomarcadores de Tumor
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Carcinoma de Pulmón de Células no Pequeñas
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Carcinoma Pulmonar de Células Pequeñas
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Biopsia por Aspiración con Aguja Fina Guiada por Ultrasonido Endoscópico
/
Neoplasias Pulmonares
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Guideline
/
Incidence_studies
/
Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Respiration
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania