Inhibition of in vitro fertilization by a monoclonal antibody reacting with the zona pellucida of the oviductal egg but not with that of the ovarian egg of the golden hamster.
J Reprod Immunol
; 14(2): 177-89, 1988 Nov.
Article
en En
| MEDLINE
| ID: mdl-2466996
ABSTRACT
A glycoprotein designated ZP-0 is secreted from the epithelial cells of the oviduct and is bound to the zonae pellucidae (ZPe) of the oviductal eggs. We have previously established a monoclonal antibody reacting with ZP-0, termed AZPO-8. In the present study, we have examined the effect of AZPO-8 on in vitro fertilization in the hamster. Treatment of the oviductal eggs with AZPO-8 at concentrations of more than 100 micrograms/ml resulted in (1) the formation of "zona precipitation" on the outer region of the ZP; (2) inhibition of sperm binding to ZP; (3) the acquisition of resistance to protease treatment. Fertilization was inhibited by AZPO-8 in a dose-dependent manner. These results indicate that a monoclonal antibody (MoAb) to an additional component of ZP derived from the oviduct shows an inhibitory effect on fertilization in vitro.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Óvulo
/
Zona Pelúcida
/
Fertilización In Vitro
/
Anticuerpos Monoclonales
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
J Reprod Immunol
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón