Identification of four lipocortin proteins and phosphorylation of lipocortin I by protein kinase C in cytosols of porcine thyroid cell cultures.
FEBS Lett
; 258(2): 346-50, 1989 Dec 04.
Article
en En
| MEDLINE
| ID: mdl-2532154
ABSTRACT
Four proteins of the lipocortin family, lipocortin I (35 kDa), lipocortin II (36 kDa), lipocortin V (32 kDa) and lipocortin VI (67-70 kDa), were identified in the cytosols of 2-day-old cultures of thyroid cells. Only lipocortin I was phosphorylated in vitro in fully differentiated, thyroid stimulating hormone-treated cells (0.1 mU/ml). Protein kinase C was the only kinase activity which phosphorylated lipocortin I. Phosphorylation shifted its pI from 6.9 to 6.6. The in vitro phosphorylation of lipocortin I was impaired in cultures exposed for 2 days to phorbol ester (10(-7) M), although it was present in both the cytosol and the particulate fraction of these cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glándula Tiroides
/
Proteína Quinasa C
/
Proteínas de Unión al Calcio
/
Glicoproteínas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1989
Tipo del documento:
Article
País de afiliación:
Francia