Assessment of intensive care unit-acquired weakness in young and old mice: An E. coli septic peritonitis model.
Muscle Nerve
; 53(1): 127-33, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26015329
ABSTRACT
INTRODUCTION:
There are few reports of in vivo muscle strength measurements in animal models of ICU-acquired weakness (ICU-AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU-AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU-AW is age-dependent in this model.METHODS:
Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined.RESULTS:
E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls.CONCLUSIONS:
This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU-AW.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Peritonitis
/
Envejecimiento
/
Debilidad Muscular
/
Escherichia coli
/
Unidades de Cuidados Intensivos
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Año:
2016
Tipo del documento:
Article
País de afiliación:
Países Bajos