Directed optimization of a newly identified squalene synthase from Mortierella alpine based on sequence truncation and site-directed mutagenesis.

Huang, Di; Yao, Yongpeng; Zhang, Hang; Mei, Zhu; Wang, Ru; Feng, Lu; Liu, Bin

Huang, Di; Yao, Yongpeng; Zhang, Hang; Mei, Zhu; Wang, Ru; Feng, Lu; Liu, Bin.

Afiliación

Huang D; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, People's Republic of China.
Yao Y; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, People's Republic of China.
Zhang H; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, People's Republic of China.
Mei Z; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, People's Republic of China.
Wang R; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, People's Republic of China.
Feng L; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, People's Republic of China.
Liu B; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, People's Republic of China.

J Ind Microbiol Biotechnol ; 42(10): 1341-52, 2015 Oct.

Article en En | MEDLINE | ID: mdl-26275528

Asunto(s)

Farnesil Difosfato Farnesil Transferasa/genética; Farnesil Difosfato Farnesil Transferasa/metabolismo; Mortierella/enzimología; Mutagénesis Sitio-Dirigida; Secuencia de Aminoácidos; Aspergillus/enzimología; Secuencia Conservada; Electroforesis en Gel de Poliacrilamida; Escherichia coli/genética; Farnesil Difosfato Farnesil Transferasa/análisis; Farnesil Difosfato Farnesil Transferasa/química; Cromatografía de Gases y Espectrometría de Masas; Mortierella/genética; Filogenia; Proteínas Recombinantes/análisis; Proteínas Recombinantes/biosíntesis; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Reishi/enzimología; Eliminación de Secuencia; Solubilidad

Palabras clave

GCMS analysis; Mortierella alpine; Site-directed mutagenesis; Squalene synthase; Truncated enzyme

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Farnesil Difosfato Farnesil Transferasa / Mutagénesis Sitio-Dirigida / Mortierella Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2015 Tipo del documento: Article

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