A set of homo-oligomeric standards allows accurate protein counting.
Angew Chem Int Ed Engl
; 54(41): 12049-52, 2015 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-26289028
ABSTRACT
Techniques based on fluorescence microscopy are increasingly used to count proteins in cells, but few stoichiometrically well-defined standards are available to test their accuracy. A selection of bacterial homo-oligomers were developed that contain 10-24 subunits and fully assemble when expressed in mammalian cells, and they can be used to easily validate/calibrate molecular counting methods. The utility of these standards was demonstrated by showing that nuclear pores contain 32â
copies of the Nup107 complex.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Poro Nuclear
/
Proteínas de Complejo Poro Nuclear
/
Synechococcus
/
Escherichia coli
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2015
Tipo del documento:
Article