Microscopy-Based High-Content Screening.
Cell
; 163(6): 1314-25, 2015 Dec 03.
Article
en En
| MEDLINE
| ID: mdl-26638068
ABSTRACT
Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, and complex organismal phenotypes. Recently, advances in imaging and image-analysis methodologies have accelerated large-scale perturbation screens. Here, we describe the state of the art for image-based screening experiments and delineate experimental approaches and image-analysis approaches as well as discussing challenges and future directions, including leveraging CRISPR/Cas9-mediated genome engineering.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Células
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Cell
Año:
2015
Tipo del documento:
Article