Cas9 Functionally Opens Chromatin.
PLoS One
; 11(3): e0152683, 2016.
Article
en En
| MEDLINE
| ID: mdl-27031353
ABSTRACT
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cromatina
/
Activación Transcripcional
/
Receptores de Ácido Retinoico
/
Proteínas Asociadas a CRISPR
/
Sistemas CRISPR-Cas
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos