Mur (MNS 10) screening with a novel loop-mediated isothermal amplification assay in Zhongshan, China.
Transfus Med
; 26(3): 215-9, 2016 Jun.
Article
en En
| MEDLINE
| ID: mdl-27102363
ABSTRACT
BACKGROUND:
The distribution of the Mur blood group antigen is 5-7% in the south of China, and a much higher prevalence is observed in some areas of the region. Anti-Mur can cause hemolytic disease of the newborn and severe transfusion reactions.OBJECTIVES:
Genetic testing is more ideal than conventional serological tests because antibodies for detection are usually not available.METHODS:
In this study, a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Mur blood group antigen was established.RESULTS:
Fifteen of 275 (5·5%) samples were confirmed by LAMP as Mur antigen positive. All the Mur antigen-positive samples were GP.Mur subtype which was confirmed with sequencing.CONCLUSION:
The LAMP method has identical results with conventional serology method but more suitable for large-scale screening.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tipificación y Pruebas Cruzadas Sanguíneas
/
Técnicas de Amplificación de Ácido Nucleico
/
Sistema del Grupo Sanguíneo MNSs
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Transfus Med
Asunto de la revista:
HEMATOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China