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Mur (MNS 10) screening with a novel loop-mediated isothermal amplification assay in Zhongshan, China.
Wei, S S; Sun, A N; Ding, S H; Meng, Q L; Wang, H M; Duan, S B; Tian, J J; Chen, Y Z; Liu, X M; Li, Y; Liao, Y T.
Afiliación
  • Wei SS; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Sun AN; Zhongshan Blood Center, Zhongshan, China.
  • Ding SH; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Meng QL; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Wang HM; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Duan SB; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Tian JJ; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Chen YZ; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Liu XM; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Li Y; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Liao YT; Zhongshan Blood Center, Zhongshan, China.
Transfus Med ; 26(3): 215-9, 2016 Jun.
Article en En | MEDLINE | ID: mdl-27102363
ABSTRACT

BACKGROUND:

The distribution of the Mur blood group antigen is 5-7% in the south of China, and a much higher prevalence is observed in some areas of the region. Anti-Mur can cause hemolytic disease of the newborn and severe transfusion reactions.

OBJECTIVES:

Genetic testing is more ideal than conventional serological tests because antibodies for detection are usually not available.

METHODS:

In this study, a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Mur blood group antigen was established.

RESULTS:

Fifteen of 275 (5·5%) samples were confirmed by LAMP as Mur antigen positive. All the Mur antigen-positive samples were GP.Mur subtype which was confirmed with sequencing.

CONCLUSION:

The LAMP method has identical results with conventional serology method but more suitable for large-scale screening.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tipificación y Pruebas Cruzadas Sanguíneas / Técnicas de Amplificación de Ácido Nucleico / Sistema del Grupo Sanguíneo MNSs Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Female / Humans / Male Idioma: En Revista: Transfus Med Asunto de la revista: HEMATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tipificación y Pruebas Cruzadas Sanguíneas / Técnicas de Amplificación de Ácido Nucleico / Sistema del Grupo Sanguíneo MNSs Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Female / Humans / Male Idioma: En Revista: Transfus Med Asunto de la revista: HEMATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China