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Molecular cloning and expression vector construction of bovine TRIM28.
Ma, X; Zhai, Z C; Zhang, M L; Song, B H; Zhu, Y R; Yang, S B; Dong, X Q; Su, L Y; Wang, C F; Ma, H X; Luan, W M.
Afiliación
  • Ma X; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Zhai ZC; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Zhang ML; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Song BH; Chang Chun Medical College, Changchun, Jilin, China.
  • Zhu YR; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Yang SB; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Dong XQ; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Su LY; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Wang CF; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Ma HX; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
  • Luan WM; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China.
Genet Mol Res ; 15(2)2016 Jun 24.
Article en En | MEDLINE | ID: mdl-27420979
ABSTRACT
The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group. The TIMR28 gene has been successfully transferred into bovine fetal fibroblasts.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Represoras Límite: Animals Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Represoras Límite: Animals Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: China