Your browser doesn't support javascript.
loading
Improving the Solubility of Artificial Ligands of Streptavidin to Enable More Practical Reversible Switching of Protein Localization in Cells.
Tachibana, Ryo; Terai, Takuya; Boncompain, Gaelle; Sugiyama, Shigeru; Saito, Nae; Perez, Franck; Urano, Yasuteru.
Afiliación
  • Tachibana R; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Terai T; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Boncompain G; Present address: Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama, 338-8570, Japan.
  • Sugiyama S; Institut Curie, Centre de Recherche, PSL Research University, 26, rue d'Ulm, Paris, 75248, France.
  • Saito N; CNRS, UMR144, PSL Research University, 26, rue d'Ulm, Paris, 75248, France.
  • Perez F; Graduate School of Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Urano Y; Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Chembiochem ; 18(4): 358-362, 2017 02 16.
Article en En | MEDLINE | ID: mdl-27905160
ABSTRACT
Chemical inducers that can control target-protein localization in living cells are powerful tools to investigate dynamic biological systems. We recently reported the retention using selective hook or "RUSH" system for reversible localization change of proteins of interest by addition/washout of small-molecule artificial ligands of streptavidin (ALiS). However, the utility of previously developed ALiS was restricted by limited solubility in water. Here, we overcame this problem by X-ray crystal structure-guided design of a more soluble ALiS derivative (ALiS-3), which retains sufficient streptavidin-binding affinity for use in the RUSH system. The ALiS-3-streptavidin interaction was characterized in detail. ALiS-3 is a convenient and effective tool for dynamic control of α-mannosidase II localization between ER and Golgi in living cells.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácidos Ftálicos / Piridonas / Sulfonamidas / Proteínas / Modelos Moleculares / Estreptavidina / Transporte de Proteínas / Ligandos Límite: Humans Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácidos Ftálicos / Piridonas / Sulfonamidas / Proteínas / Modelos Moleculares / Estreptavidina / Transporte de Proteínas / Ligandos Límite: Humans Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Japón