Finding sRNA-associated phenotypes by competition assays: An example with Staphylococcus aureus.
Methods
; 117: 21-27, 2017 03 15.
Article
en En
| MEDLINE
| ID: mdl-27916561
ABSTRACT
Bacteria optimize their fitness in response to a changing environment by tight regulation of gene expression. Regulation can be controlled at both transcriptional and post-transcriptional levels via key players such as sigma factors, regulatory proteins and regulatory RNAs. The identification of phenotypes associated with gene deletions is the established method for finding gene functions but may require testing many conditions for each studied mutant. As regulatory RNAs often contribute to fine-tuning gene expression, phenotypes associated with their inactivation are often weak and difficult to detect. Nevertheless, minor phenotypes conferring modest advantages, may allow bacteria to emerge after some generations under selective pressure. A strategy employing DNA barcodes can be used to perform competition experiments between mutants and to monitor fitness associated with mutations in different growth conditions. We combined this strategy with deep sequencing to study regulatory RNAs in Staphylococcus aureus, a major opportunistic pathogen.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
/
Bioensayo
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ARN Bacteriano
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Regulación Bacteriana de la Expresión Génica
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Interacciones Microbianas
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ARN Pequeño no Traducido
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Francia