Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide.
Int Endod J
; 51 Suppl 2: e115-e124, 2018 Feb.
Article
en En
| MEDLINE
| ID: mdl-28333374
ABSTRACT
AIM:
To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC-23 cells. It was hypothesized that MDPC-23 odontoblast-like cells may function as odontoclasts under the influence of LPS.METHODOLOGY:
MDPC-23 cells were cultured in the presence of 0.1 or 1 µg mL-1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast-like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t-test and one-way anova were used in statistical analysis.RESULTS:
TRAP-positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 µg mL-1 LPS-induced cells (P < 0.05). Osteoclast-specific proteins such as TRAP cathepsin K and Rac1 were upregulated in the 1 µg mL-1 LPS-treated cells (P < 0.05), whilst the expression of marker proteins of the RANKL-RANK signalling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (P > 0.05). ROS production was observed in the 1 µg mL-1 LPS treatment group (P < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS-treated group and the control group (P > 0.05).CONCLUSIONS:
A known value of 1 µg mL-1 LPS might induce odontoblast-like MDPC-23 cells to generate odontoclast-like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Lipopolisacáridos
/
Papila Dental
/
Odontogénesis
Límite:
Animals
Idioma:
En
Revista:
Int Endod J
Año:
2018
Tipo del documento:
Article
País de afiliación:
China