Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells.
Biochem Biophys Res Commun
; 487(2): 344-350, 2017 05 27.
Article
en En
| MEDLINE
| ID: mdl-28412348
ABSTRACT
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Separación Celular
/
Islotes Pancreáticos
/
Células Madre Pluripotentes
/
Dispositivos Laboratorio en un Chip
/
Técnicas de Cultivo Celular por Lotes
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2017
Tipo del documento:
Article