Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples.
Methods Mol Biol
; 1726: 77-84, 2018.
Article
en En
| MEDLINE
| ID: mdl-29468545
ABSTRACT
The retinoblastoma protein (pRb) is an important tumor suppressor and cell cycle repressor. pRb is a phosphoprotein whose function is regulated primarily at the level of phosphorylation, and therefore, detecting pRb's phosphorylation status in human tissue samples can be clinically informative. Unfortunately, detection of phosphorylated pRb residues can be technically challenging, as these residues can often be weak antigens. In this chapter, we describe an enhanced sensitivity immunohistochemistry protocol for the staining of phosphorylated serine 249 in pRb, in human lung tumor samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas para Inmunoenzimas
/
Ubiquitina-Proteína Ligasas
/
Proteínas de Unión a Retinoblastoma
/
Neoplasias Pulmonares
Tipo de estudio:
Diagnostic_studies
/
Guideline
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Puerto Rico