Amycolatopsis silviterrae sp. nov., isolated from forest soil.
Int J Syst Evol Microbiol
; 68(5): 1455-1460, 2018 May.
Article
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| MEDLINE
| ID: mdl-29533177
ABSTRACT
A novel actinobacterial strain, designated C12CA1T, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1T contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1T was classified in the genus Amycolatopsis. It contained MK-9(H4) as the predominant menaquinone, C16ââ0, iso-C15ââ0 and iso-C16ââ0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1T was closely related to Amycolatopsis vancoresmycina DSM 44592T (98.96â%) and Amycolatopsis pretoriensis JCM 12673T (98.82â%). The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592T (6.9±0.2-11.6±1.9â%) and A. pretoriensis JCM 12673T (8.8±0.3-9.2±1.8â%). The DNA G+C content of strain C12CA1T was 69.8 mol%. Based on the results of polyphasic characterization, strain C12CA1T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp. nov. is proposed. The type strain is C12CA1T (=TBRC 1456T=NBRC 111116T).
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Microbiología del Suelo
/
Actinomycetales
/
Bosques
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Tailandia