Adipogenesis in Primary Cell Culture.
Handb Exp Pharmacol
; 251: 73-84, 2019.
Article
en En
| MEDLINE
| ID: mdl-29980911
ABSTRACT
Obesity involves a contrasting expansion of the energy-storing white fat and loss of functionally competent brown fat, an energy-consuming thermogenic adipose. Leveraging our understanding of white and brown adipocyte recruitment and investigating factors that regulate these processes might reveal novel targets for counteracting obesity. In vitro differentiation of primary preadipocytes mimics many of the morphological and transcriptional events occurring during adipogenesis in vivo. Moreover, preadipocytes isolated from a specific depot maintain features of their originating niche. This makes in vitro adipogenesis a valuable model for identifying differential regulation patterns between brown and white adipogenesis. In this chapter, we describe step-by-step how to isolate brown and white preadipocytes from human tissue biopsies and how to culture and differentiate them in vitro. We discuss this process, what to consider, and how this in vitro system can be used to model in vivo adipogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tejido Adiposo Pardo
/
Adipogénesis
/
Tejido Adiposo Blanco
/
Adipocitos Marrones
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Handb Exp Pharmacol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Dinamarca