Use of Real-Time Polymerase Chain Reaction to Differentiate between Pathogenic Entamoeba histolytica and the Nonpathogenic Entamoeba dispar in Ecuador.
Am J Trop Med Hyg
; 100(1): 81-82, 2019 01.
Article
en En
| MEDLINE
| ID: mdl-30398142
ABSTRACT
Microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of Entamoeba histolytica. Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for E. histolytica/Entamoeba dispar by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for E. dispar and only three (2.8%) were positive for E. histolytica. Some 29 (27.4%) samples were negative for the presence of either E. histolytica or E. dispar, this may be due the presence of Entamoeba mosksvskii, which is morphologically identical to E. histolytica/E. dispar and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Diagnóstico Molecular
/
Entamoeba
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Entamoeba histolytica
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Entamebiasis
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Reacción en Cadena en Tiempo Real de la Polimerasa
/
Parasitosis Intestinales
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Ecuador
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2019
Tipo del documento:
Article
País de afiliación:
Ecuador