MicroRNA-140-5p aggravates hypertension and oxidative stress of atherosclerosis via targeting Nrf2 and Sirt2.
Int J Mol Med
; 43(2): 839-849, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30483753
ABSTRACT
In the present study, the function of microRNA (miR)1405p on oxidative stress in mice with atherosclerosis was investigated. A reverse transcriptionquantitative polymerase chain reaction assay was used to determine the expression of miR1405p. Oxidative stress kits and reactive oxygen species (ROS) kits were used to analyze alterations in oxidative stress and ROS levels. The alterations in protein expression were determined using western blot analysis and an immunofluorescence assay. miR1405p expression was increased in mice with atherosclerosis with hypertension. Consistently, miR1405p expression was also increased in mice with atherosclerosis. Upregulation of miR1405p increased oxidative stress and ROS levels by suppressing the protein expression of nuclear factor erythroid 2related factor 2 (Nrf2), sirtuin 2 (Sirt2), Kelchlike enoylCoA hydrataseassociated protein 1 (Keap1) and heme oxygenase 1 (HO1) in vitro. By contrast, downregulation of miR1405p decreased oxidative stress and ROS levels by activating the protein expression of Nrf2, Sirt2, Keap1 and HO1 in vitro. Sirt2 agonist or Nrf2 agonist inhibited the effects of miR1405p on oxidative stress in vitro. Collectively, these results suggested that miR1405p aggravated hypertension and oxidative stress of mice with atherosclerosis by targeting Nrf2 and Sirt2.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
MicroARNs
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Aterosclerosis
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Hipertensión
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Int J Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
2019
Tipo del documento:
Article