Estrogen receptor ß plays a protective role in zearalenone-induced oxidative stress in normal prostate epithelial cells.
Ecotoxicol Environ Saf
; 172: 504-513, 2019 May 15.
Article
en En
| MEDLINE
| ID: mdl-30738973
ABSTRACT
Zearalenone (ZEA) - a fungal mycotoxin is reported to both cause the oxidative stress associated with death of cells as well as induction of the proliferation of cells, depending on its concentration and the type of cells. ZEA due to its structural similarity to naturally occurring estrogens is able to bind to estrogen receptors and triggers estrogen-associated signaling pathways. The aim of this study is to evaluate whether the induction of oxidative stress in normal epithelial prostate PNT1A cells is associated with estrogenic activity of ZEA. We observed that ZEA-induced oxidative stress in PNT1A cells is associated with a decrease in the oxidative stress defense enzymes expression, cell cycle arrest in G2/M cell cycle phase as well as the decreased migration of cells. The results also suggest that the observed effect might be associated with the nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)- hypoxia inducible factor 1 alpha (HIF-1α) signaling pathway. The usage of estrogen receptor ß (ERß) selective antagonist 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]-phenol PHTPP showed that ERß activity is able to decrease the ZEA-induced oxidative stress, but is not enough to counteract it, indicating that ZEA-induced oxidative stress is only partially associated with estrogenic activity of ZEA.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Próstata
/
Zearalenona
/
Estrés Oxidativo
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Receptor beta de Estrógeno
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Células Epiteliales
/
Puntos de Control del Ciclo Celular
Límite:
Humans
/
Male
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2019
Tipo del documento:
Article