Phosphofructokinase-1 subunit composition and activity in the skeletal muscle, liver, and brain of dogs.
J Vet Med Sci
; 81(5): 712-716, 2019 May 31.
Article
en En
| MEDLINE
| ID: mdl-30918224
ABSTRACT
Phosphofructokinase-1 (EC2.7.1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme of glycolysis. Mammalian PFK-1 isozymes are composed of three kinds of subunits (PFK-M, -L, and -P), with different properties. It has been suggested that the proportion of PFK-1 subunits in different organs is based on the organ energy metabolism. In this study, we analyzed the activity and subunit composition of canine PFK-1. We found that, in dogs, the skeletal muscle only has PFK-M, the liver mainly has PFK-L, and the brain expresses all of them. The knowledge of the composition of PFK-1 could provide useful information for determination of the differences in glycolysis in various organs of dogs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfofructoquinasa-1
/
Perros
/
Isoenzimas
Límite:
Animals
Idioma:
En
Revista:
J Vet Med Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón