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Genomic characterisation of a novel plasmid carrying blaIMP-6 of carbapenem-resistant Klebsiella pneumoniae isolated in Osaka, Japan.
Abe, Ryuichiro; Akeda, Yukihiro; Sakamoto, Noriko; Kumwenda, Geoffrey; Sugawara, Yo; Yamamoto, Norihisa; Kawahara, Ryuji; Tomono, Kazunori; Fujino, Yuji; Hamada, Shigeyuki.
Afiliación
  • Abe R; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; Department of Anaesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.
  • Akeda Y; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; Division of Infection Control and Prevention, Osaka University Hospital, Osaka University, Suita, Japan. Electronic address: akeda@biken.osa
  • Sakamoto N; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
  • Kumwenda G; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
  • Sugawara Y; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
  • Yamamoto N; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; Division of Infection Control and Prevention, Osaka University Hospital, Osaka University, Suita, Japan.
  • Kawahara R; Department of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
  • Tomono K; Division of Infection Control and Prevention, Osaka University Hospital, Osaka University, Suita, Japan.
  • Fujino Y; Department of Anaesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.
  • Hamada S; Thailand-Japan Research Collaboration Centre on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
J Glob Antimicrob Resist ; 21: 195-199, 2020 06.
Article en En | MEDLINE | ID: mdl-31627024
OBJECTIVES: To analyse plasmids carrying blaIMP-6 in Klebsiella pneumoniae isolates obtained from multicentre carbapenem-resistant Enterobacteriaceae surveillance. METHODS: Plasmids harbouring blaIMP-6 were characterised by the whole-genome sequencing of four Klebsiella pneumoniae isolates carrying blaIMP-6, and compared with the pKPI-6 plasmid, which is widespread in western Japan, through pulsed-field gel electrophoresis, Southern blotting, bacterial conjugation, and qPCR. RESULTS: Whole-genome sequencing analysis revealed that three of the four isolates carried approximately 50 kbp plasmids similar to the pKPI-6 plasmid; however, one isolate carried a 250 kbp plasmid harbouring blaIMP-6 (pE196_IMP6). So far, all of the reported plasmids carrying blaIMP-6 were similar to the pKPI-6 plasmid, and this plasmid was a novel blaIMP6-carrier. The size and transferability of this plasmid was confirmed by Southern hybridisation and conjugation experiments. It was demonstrated that the generation of plasmid pE196_IMP6 was due to an intramolecular transposition mediated by IS26, and a homologous recombination between plasmids pKPI-6 and pE013 that was obtained from another carbapenem-resistant Enterobacteriaceae isolate in this analysis. As a result of co-integration with pE013, pE196_IMP6 acquired six additional pairs of type II toxin-antitoxin systems that pKPI-6 does not carry. Transcription of all of the toxin-antitoxin systems were confirmed in an isolate carrying pE196_IMP6 by qPCR. CONCLUSIONS: This study detected a novel plasmid carrying blaIMP-6, and revealed the origin of this plasmid. Toxin-antitoxin system acquisition could enable pE196_IMP6 maintenance persistently through successions, even without selection pressure by the clinical usage of antimicrobials, generating broad dissemination and longer carbapenem-resistant Enterobacteriaceae colonisation duration in patients.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enterobacteriaceae Resistentes a los Carbapenémicos / Klebsiella pneumoniae Tipo de estudio: Clinical_trials Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Glob Antimicrob Resist Año: 2020 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enterobacteriaceae Resistentes a los Carbapenémicos / Klebsiella pneumoniae Tipo de estudio: Clinical_trials Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Glob Antimicrob Resist Año: 2020 Tipo del documento: Article País de afiliación: Japón