The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation.

Zhang, Qian; Wen, Fengcai; Zhang, Siqi; Jin, Jiachuan; Bi, Lulu; Lu, Ying; Li, Ming; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin; Sun, Bo

Zhang, Qian; Wen, Fengcai; Zhang, Siqi; Jin, Jiachuan; Bi, Lulu; Lu, Ying; Li, Ming; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin; Sun, Bo.

Afiliación

Zhang Q; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Wen F; Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Zhang S; University of Chinese Academy of Sciences, Beijing 100049, China.
Jin J; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Bi L; Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Lu Y; University of Chinese Academy of Sciences, Beijing 100049, China.
Li M; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Xi XG; Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Huang X; University of Chinese Academy of Sciences, Beijing 100049, China.
Shen B; State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing 211166, China.
Sun B; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Sci Adv ; 5(11): eaaw9807, 2019 11.

Article en En | MEDLINE | ID: mdl-31763447

Asunto(s)

Proteína 9 Asociada a CRISPR/metabolismo; Sistemas CRISPR-Cas; ADN/metabolismo; ARN Guía de Kinetoplastida/metabolismo; Proteína 9 Asociada a CRISPR/genética; ADN/química; ADN/genética; Edición Génica/métodos; Modelos Genéticos; Conformación de Ácido Nucleico; Motivos de Nucleótidos/genética; Unión Proteica; ARN Guía de Kinetoplastida/química; ARN Guía de Kinetoplastida/genética; Análisis de Secuencia de ADN/métodos

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / ARN Guía de Kinetoplastida / Sistemas CRISPR-Cas / Proteína 9 Asociada a CRISPR Idioma: En Revista: Sci Adv Año: 2019 Tipo del documento: Article País de afiliación: China

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