Cell-Wide Survey of Amide-Bonded Lysine Modifications by Using Deacetylase CobB.
Biol Proced Online
; 21: 23, 2019.
Article
en En
| MEDLINE
| ID: mdl-31798349
ABSTRACT
BACKGROUND:
Lysine post-translational modifications are important regulators of protein function. Proteomic and biochemical approaches have resulted in identification of several lysine modifications, including acetylation, crotonylation, and succinylation. Here, we developed an approach for surveying amide-bonded lysine modifications in the proteome of human tissues/cells based on the observation that many lysine modifications are amide-bonded and that the Salmonella enterica deacetylase, CobB, is an amidase.RESULTS:
After the proteome of human tissues/cells was denatured and the non-covalently bonded metabolites were removed by acetone washes, and the amide-bonded modifiers were released by CobB and analyzed using liquid- and/or gas chromatography/mass spectrometry metabolomic analysis. This protocol, which required 3-4 days for completion, was used to qualitatively identify more than 40 documented and unreported lysine modifications from the human proteome and to quantitatively analyze dynamic changes in targeted amide-bonded lysine modifications.CONCLUSIONS:
We developed a method that was capable of monitoring and quantifying amide-bonded lysine modifications in cells of different origins.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Biol Proced Online
Año:
2019
Tipo del documento:
Article