A de novo peroxidase is also a promiscuous yet stereoselective carbene transferase.
Proc Natl Acad Sci U S A
; 117(3): 1419-1428, 2020 01 21.
Article
en En
| MEDLINE
| ID: mdl-31896585
ABSTRACT
By constructing an in vivo-assembled, catalytically proficient peroxidase, C45, we have recently demonstrated the catalytic potential of simple, de novo-designed heme proteins. Here, we show that C45's enzymatic activity extends to the efficient and stereoselective intermolecular transfer of carbenes to olefins, heterocycles, aldehydes, and amines. Not only is this a report of carbene transferase activity in a completely de novo protein, but also of enzyme-catalyzed ring expansion of aromatic heterocycles via carbene transfer by any enzyme.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Peroxidasas
/
Proteínas de Escherichia coli
/
Biocatálisis
/
Metano
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido