Improved in-solution trypsin digestion method for methanol-chloroform precipitated cellular proteomics sample.

Shahinuzzaman, A D A; Chakrabarty, Jayanta K; Fang, Zixiang; Smith, David; Kamal, Abu Hena Mostafa; Chowdhury, Saiful M

Shahinuzzaman, A D A; Chakrabarty, Jayanta K; Fang, Zixiang; Smith, David; Kamal, Abu Hena Mostafa; Chowdhury, Saiful M.

Afiliación

Shahinuzzaman ADA; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.
Chakrabarty JK; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.
Fang Z; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.
Smith D; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.
Kamal AHM; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.
Chowdhury SM; Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas, USA.

J Sep Sci ; 43(11): 2125-2132, 2020 Jun.

Article en En | MEDLINE | ID: mdl-32073721

Asunto(s)

Cloroformo/metabolismo; Metanol/metabolismo; Proteómica; Tripsina/análisis; Tripsina/metabolismo; Animales; Bicarbonatos/química; Bicarbonatos/metabolismo; Cloroformo/química; Cromatografía Liquida; Metanol/química; Ratones; Células RAW 264.7; Soluciones; Espectrometría de Masas en Tándem

Palabras clave

deoxycholic acid; methanol-chloroform precipitation; proteomics sample preparation; raw macrophages; trypsin digestion

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tripsina / Cloroformo / Proteómica / Metanol Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: J Sep Sci Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

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