A Water-soluble Near-infrared Fluorescent Probe for Cysteine/Homocysteine and Its Application in Live Cells and Mice.
Anal Sci
; 36(9): 1053-1057, 2020 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-32115466
ABSTRACT
A near-infrared (NIR) and water-soluble probe was synthesized and studied for the detection of Cys/Hcy in aqueous solution, living cells and mice. The probe was composed of cyanine derivative as the NIR fluorescent reporting unit and pyrimidiny-thioether moiety as the Cys/Hcy responsive unit. Treatment with Cys/Hcy induced the formation of sulfur-substituted products, then intramolecular rearrangement reaction would occur to produce amino-substituted products and resulting in enhanced red fluorescence emissions. It could be applied to sense Cys/Hcy both in solution with the detection limit of 0.17 µM (or 0.32 µM) and in living cells. Cell imaging experiments proved that such a probe exhibited good cell penetration. In addition, the probe could detect Cys/Hcy in live mice with strong turn-on fluorescent response.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Agua
/
Cisteína
/
Colorantes Fluorescentes
/
Homocisteína
/
Rayos Infrarrojos
Límite:
Animals
Idioma:
En
Revista:
Anal Sci
Año:
2020
Tipo del documento:
Article
País de afiliación:
China