Overexpression and Biochemical Characterization of an Endo-&#945;-1,4-polygalacturonase from <i>Aspergillus nidulans</i> in <i>Pichia pastoris</i>.

Xu, Hua; Zhang, Pengfei; Zhang, Yuchen; Liu, Zebin; Zhang, Xuebing; Li, Zhimin; Li, Jian-Jun; Du, Yuguang

Overexpression and Biochemical Characterization of an Endo-α-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris.

Xu, Hua; Zhang, Pengfei; Zhang, Yuchen; Liu, Zebin; Zhang, Xuebing; Li, Zhimin; Li, Jian-Jun; Du, Yuguang.

Afiliación

Xu H; Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang 330045, China.
Zhang P; Sichuan Normal University, College of Life Science, Chengdu 610101, China.
Zhang Y; National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Liu Z; Capital Normal University, College of Life Sciences, Beijing 10048, China.
Zhang X; National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Li Z; Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang 330045, China.
Li JJ; National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Du Y; National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

Int J Mol Sci ; 21(6)2020 Mar 19.

Article en En | MEDLINE | ID: mdl-32204337

Asunto(s)

Aspergillus nidulans/genética; Proteínas Fúngicas/genética; Pichia/genética; Poligalacturonasa/genética; Aspergillus nidulans/enzimología; Estabilidad de Enzimas; Proteínas Fúngicas/química; Proteínas Fúngicas/metabolismo; Regulación Enzimológica de la Expresión Génica; Regulación Fúngica de la Expresión Génica; Concentración de Iones de Hidrógeno; Cinética; Modelos Moleculares; Filogenia; Poligalacturonasa/clasificación; Poligalacturonasa/metabolismo; Conformación Proteica; Especificidad por Sustrato; Temperatura

Palabras clave

Aspergillus nidulans; endo-α-1,4-polygalacturonase; identification of hydrolysis products; pH and temperature-rate profile; pH and thermal stability; structure modelling

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pichia / Poligalacturonasa / Aspergillus nidulans / Proteínas Fúngicas Tipo de estudio: Prognostic_studies Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: China

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