Intracellular pH in platelets from spontaneously hypertensive rats and Wistar-Kyoto rats.
J Hypertens Suppl
; 6(4): S252-4, 1988 Dec.
Article
en En
| MEDLINE
| ID: mdl-3241212
ABSTRACT
Intracellular pH in platelets from spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto rats (WKY) was measured using a pH-sensitive fluorescent dye, 2',7'-bis(carboxyethyl)carboxyfluorescein tetraacetoxy methyl ester (BCECF). Intracellular pH in platelets was significantly higher in SHR than in WKY (7.12 +/- 0.04 versus 7.06 +/- 0.02, P less than 0.01; n = 10). There was a close correlation between intracellular pH and systolic blood pressure (r = 0.66, P less than 0.01). Addition to platelets of 1-(5-isoquinolinesulphonyl)-2-methylpiperazine (H-7), a specific inhibitor of protein kinase C, produced a significantly larger decrease in intracellular pH in SHR than in WKY (0.06 +/- 0.02 versus 0.03 +/- 0.00, P less than 0.01, n = 5). These results suggest that protein kinase C may play an important role in the increased intracellular pH in SHR.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ratas Endogámicas SHR
/
Ratas Endogámicas
/
Sulfonamidas
/
Plaquetas
/
Proteína Quinasa C
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Concentración de Iones de Hidrógeno
/
Hipertensión
Límite:
Animals
Idioma:
En
Revista:
J Hypertens Suppl
Asunto de la revista:
ANGIOLOGIA
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón