Oral HPV prevalence assessment by Linear Array vs. SPF10 PCR-DEIA-LiPA25 system in the HPV Infection in Men (HIM) study.
Papillomavirus Res
; 9: 100199, 2020 06.
Article
en En
| MEDLINE
| ID: mdl-32464335
ABSTRACT
INTRODUCTION:
Oral human papillomavirus (HPV) attributable oropharyngeal cancers are on the rise in many countries. Oral HPV infections among healthy individuals are commonly detected using oral gargle samples. However, the optimal method for HPV genotyping oral gargle specimens in research studies has not been previously evaluated. MATERIALS ANDMETHODS:
Oral gargle samples from 1455 HPV Infection in Men (HIM) study participants were HPV genotyped using two differentmethods:
Linear Array and the SPF10 PCR-DEIA-LiPA25. The sensitivity of the two tests for detecting individual HPV types and grouped HPV types, high-risk HPV, low-risk HPV, grouped 4-HPV-vaccine types, and grouped 9-HPV-vaccine-types, and the degree of concordance between the two tests was assessed. We also examined whether socio-demographic-behavioral factors were associated with concordance between the two assays.RESULTS:
The sensitivity of SPF10 PCR-DEIA-LiPA25 was higher than Linear Array, with the exception of HPV 70, for the detection of oral HPV. The prevalence ratio of SPF10 PCR-DEIA-LiPA25 to Linear Array varied between 1.0 and 9.0 for individual HPV genotypes, excluding HPV 70, and between 3.8 and 4.4 for grouped 4-valent and 9-valent HPV vaccine types, respectively. There was no association between socio-demographic-behavioral factors and discordance in results between the two tests for oral HPV 16 detection.DISCUSSION:
SPF10 PCR-DEIA-LiPA25 was more sensitive than Linear Array for detecting HPV in oral gargle samples. Given the growing importance of detecting oral HPV infection for research studies of oral HPV natural history and vaccine effectiveness evaluation, we recommend using methods with higher sensitivity such as SPF10 PCR-DEIA-LiPA25 for detecting HPV in oral gargle samples.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Infecciones por Papillomavirus
/
Alphapapillomavirus
/
Boca
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Prevalence_studies
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Risk_factors_studies
Límite:
Adolescent
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Adult
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Aged
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
America do sul
/
Brasil
/
Mexico
Idioma:
En
Revista:
Papillomavirus Res
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos