QTL mapping of web blotch resistance in peanut by high-throughput genome-wide sequencing.
BMC Plant Biol
; 20(1): 249, 2020 Jun 03.
Article
en En
| MEDLINE
| ID: mdl-32493219
ABSTRACT
BACKGROUND:
Web blotch is one of the most important foliar diseases worldwide in peanut (Arachis hypogaea L.). The identification of quantitative trait loci (QTLs) for peanut web blotch resistance represents the basis for gene mining and the application of molecular breeding technologies.RESULTS:
In this study, a peanut recombinant inbred line (RIL) population was used to map QTLs for web blotch resistance based on high-throughput genome-wide sequencing. Frequency distributions of disease grade and disease index in five environments indicated wide phenotypic variations in response to web blotch among RILs. A high-density genetic map was constructed, containing 3634 bin markers distributed on 20 peanut linkage groups (LGs) with an average genetic distance of 0.5 cM. In total, eight QTLs were detected for peanut web blotch resistance in at least two environments, explaining from 2.8 to 15.1% of phenotypic variance. Two major QTLs qWBRA04 and qWBRA14 were detected in all five environments and were linked to 40 candidate genes encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) or other proteins related to disease resistances.CONCLUSIONS:
The results of this study provide a basis for breeding peanut cultivars with web blotch resistance.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arachis
/
Enfermedades de las Plantas
/
Sitios de Carácter Cuantitativo
/
Resistencia a la Enfermedad
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
BMC Plant Biol
Asunto de la revista:
BOTANICA
Año:
2020
Tipo del documento:
Article