Flavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa.
Int J Syst Evol Microbiol
; 70(6): 3888-3898, 2020 Jun.
Article
en En
| MEDLINE
| ID: mdl-32496180
ABSTRACT
A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5T, was isolated from rhizosphere soil of Suaeda salsa, in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed that strain XS-5T clustered with Flavobacterium beibuense F44-8T (shared 97.2â% of 16S rRNA gene similarity) and Flavobacterium rakeshii FCS-5T (97.6â%), and shared <96.0â% of 16S rRNA gene similarities with all other type strains. Strain XS-5T contained MK-6 as the major respiratory quinone. Its major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid; and the major fatty acids were iso-C15ââ0, iso-C17ââ0 3-OH, C16ââ0, iso-C15ââ0 3-OH, Summed feature 3 (iso-C15ââ0 2-OH and/or C16ââ1 ω7c), and Summed feature 9 (iso-C17ââ1 ω9c and/or C16ââ0 10-methyl). The genome consisted of a 3â985â855 bp circular chromosome, with a G+C content of 37.9 mol%, predicting 3616 coding sequences genes, 45 tRNA genes and three rRNA operons. The average nucleotide identity, amino acid identity and digital DNA-DNA hybridization values of strain XS-5T to F. beibuense F44-8T and F. rakeshii FCS-5T were 79.2 and 79.2â%, 81.7 and 81.6 %, 22.3 and 22.2â%, respectively. The results of phylogenetic, physiological and biochemical tests allowed the discrimination of strain XS-5T from its phylogenetic relatives. Flavobacterium alkalisoli sp. nov. is therefore proposed with strain XS-5T (=CGMCC 1.17077T=KCTC 72459T) as the type strain.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Microbiología del Suelo
/
Flavobacterium
/
Chenopodiaceae
/
Rizosfera
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2020
Tipo del documento:
Article