IL-10-1082G>A polymorphism, use of opioids and age affect the course of acute pancreatitis.

ABSTRACT

PURPOSE: We aimed to determine the association of two of the most important functional polymorphisms of IL-8 and IL-10 with the clinical course and outcome of acute pancreatitis. METHOD: Ninety-three patients with acute pancreatitis were genotyped for IL-8-251T>A and IL-10-1082G>A using PCR-RFLP. The severity of the disease was determined based on the Atlanta Classification system. RESULTS: In patients treated with opioids, the odds for severe form of acute pancreatitis, its complications, and death were increased. Advanced age was associated with higher odds of organ/multiple organ failure and other systemic complications. Multivariate logistic regression analyses confirmed the observed effect of age and use of opioids, and revealed higher odds for the development of severe form of acute pancreatitis [P = 0.017, odds ratio (OR) 4.324, 95% confidence interval (CI) 1.305-14.323], its complications in general (P = 0.011, OR 4.936, 95% CI 1.442-16.897), pancreatic necrosis (P = 0.032, OR 3.922, 95% CI 1.122-13.707) and systemic inflammatory response syndrome (P = 0.037, OR 3.838, 95% CI 1.085-13.583) in the absence of IL-10-1082G>A variant allele. The effect of IL-8 -251T>A on acute pancreatitis severity or mortality was not detected. CONCLUSION: Our study suggests the IL-10 -1082A allele as a protective factor in acute pancreatitis. Opioid analgesics treatment in acute pancreatitis is associated with severity, complications and mortality, while advanced age increases the risk of systemic complications.