Adding to the CASeload: unwarranted p53 signaling induced by Cas9.
Mol Cell Oncol
; 7(5): 1789419, 2020.
Article
en En
| MEDLINE
| ID: mdl-32944644
ABSTRACT
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Mol Cell Oncol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos