Sensitivity of human papillomaviruspositive and negative oropharyngeal cancer cell lines to ionizing irradiation.
Oncol Rep
; 44(4): 1717-1726, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32945506
ABSTRACT
Human papillomaviruspositive (HPV+) oropharyngeal squamous cell carcinoma (OPSCC) has increased in incidence and has a much better prognosis than HPVnegative (HPV) OPSCC with radiotherapy alone, but exactly why is unknown. The present study therefore aimed to further examine the sensitivity and possible changes in gene expression of several HPV+ and HPV OPSCC, including various novel cell lines, upon ionizing irradiation (IR). Previously established HPV+ UMSCC47, UPCISCC90, CUOP2, CUOP3 and HPV UMSCC4, UMSCC6, UMSCC74a, UMSCC19 and newly established CUOP17 and CUOP20, characterised here, were subjected to 06 Gy. Surviving fractions of each cell line were tested by clonogenic assays, and irregularities in cell cycle responses were examined by flow cytometry, while changes in gene expression were followed by mRNA sequencing. HPV+ OPSCC cell lines showed greater variation in sensitivity to ionizing irradiation (IR) and tended to be more sensitive than HPV OPSCC cell lines. However, their IR sensitivity was not correlated to the proportion of cells in G2 arrest, and HPV cell lines generally showed lower increases in G2 after IR. Upon IR with 2 Gy, mRNA sequencing revealed an increase in minor HPV integration sites in HPV+ cell lines, and some changes in gene expression in OPSCC cell lines, but not primarily those associated with DNA repair. To conclude, HPV+ OPSCC cell lines showed greater variation in their sensitivity to IR, with some that were radioresistant, but overall the HPV+ OPSCC group still tended to be more sensitive to IR than the HPV OPSCC group. In addition, HPV+ OPSCC lines were more frequently in G2 as compared to HPV cell lines, but the increase in G2 arrest upon IR in HPV+ OPSCC was not correlated to sensitivity to IR. Increases in minor HPV integration sites and changes in gene expression were also demonstrated after irradiation with 2 Gy.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tolerancia a Radiación
/
Infecciones por Papillomavirus
/
Carcinoma de Células Escamosas de Cabeza y Cuello
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido