SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells.
Sci Rep
; 10(1): 15805, 2020 09 25.
Article
en En
| MEDLINE
| ID: mdl-32978492
ABSTRACT
Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new protocol based on surface enhanced resonance Raman scattering (SERRS) gold multivalent nanostructures to identify and enumerate tumor cells with epithelial and mesenchimal markers. The validation of the protocol is obtained with spiked samples of peripheral blood mononuclear cells (PBMC). Gold nanostructures are functionalized with SERRS labels and with antibodies to link the tumor cells. Three types of such nanosystems were simultaneously used and the protocol allows obtaining the identification of all individual tumor cells with the help of a Random Forest ensemble learning method.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Espectrometría Raman
/
Leucocitos Mononucleares
/
Glioblastoma
/
Nanoestructuras
/
Células Epiteliales
/
Células Madre Mesenquimatosas
Tipo de estudio:
Diagnostic_studies
/
Guideline
Límite:
Humans
/
Male
Idioma:
En
Revista:
Sci Rep
Año:
2020
Tipo del documento:
Article
País de afiliación:
Italia